<?xml version="1.0" encoding="UTF-8" ?><!-- generator=Zoho Sites --><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom" xmlns:content="http://purl.org/rss/1.0/modules/content/"><channel><atom:link href="https://www.biofilmengineers.in/blogs/Beyond-treatment/feed" rel="self" type="application/rss+xml"/><title>Biofilm Engineers - Blog - Let's explore wastewater , Beyond treatment</title><description>Biofilm Engineers - Blog - Let's explore wastewater , Beyond treatment</description><link>https://www.biofilmengineers.in/blogs/Beyond-treatment</link><lastBuildDate>Sat, 13 Jun 2026 21:17:44 +0530</lastBuildDate><generator>http://zoho.com/sites/</generator><item><title><![CDATA[Paneer Makes the Profit. But What Happens to the Whey? The Untold Story Behind Dairy Wastewater Treatment]]></title><link>https://www.biofilmengineers.in/blogs/post/paneer-makes-the-profit.-but-what-happens-to-the-whey-the-untold-story-behind-dairy-wastewater-treat</link><description><![CDATA[A family of five gathers around the dinner table after a long day. The aroma of fresh paneer fills the room. “Paneer butter masala today?” the younges ]]></description><content:encoded><![CDATA[<div class="zpcontent-container blogpost-container "><div data-element-id="elm_cuKfuKugQT6q-So5nOf6zg" data-element-type="section" class="zpsection "><style type="text/css"></style><div class="zpcontainer-fluid zpcontainer"><div data-element-id="elm_uCkwrslZTeugGp1OklX__w" data-element-type="row" class="zprow zprow-container zpalign-items- zpjustify-content- " data-equal-column=""><style type="text/css"></style><div data-element-id="elm_XyBdGnEeREuabAtWmiWuRQ" data-element-type="column" class="zpelem-col zpcol-12 zpcol-md-12 zpcol-sm-12 zpalign-self- "><style type="text/css"></style><div data-element-id="elm_8pN9zpAsT3qVm-KULKPbnA" data-element-type="heading" class="zpelement zpelem-heading "><style></style><h2
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<div data-element-id="elm_1bc27uQIQfCft-zS2-Dnrg" data-element-type="text" class="zpelement zpelem-text "><style></style><div class="zptext zptext-align-center zptext-align-mobile-center zptext-align-tablet-center " data-editor="true"><p></p><div><p style="text-align:left;"><img src="/Blog%204%20Panner%20Whey%20ETP.png"/><span></span></p><p style="text-align:left;"><span><br/></span></p><p style="text-align:left;"><span>A family of five gathers around the dinner table after a long day. The aroma of fresh paneer fills the room.</span></p><p style="text-align:left;"><span>“Paneer butter masala today?” the youngest asks excitedly.</span></p><p style="text-align:left;"><span>Alongside it sits paneer tikka, kadai paneer, shahi paneer, and soft paneer stuffed parathas. The family laughs, eats, and enjoys every bite.</span></p><p style="text-align:left;"><span>“This is the softest paneer I’ve ever had,” says the grandfather with a smile.</span></p><p style="text-align:left;"><span>The dinner ends happily. Plates are cleared. Lights go off.</span></p><p style="text-align:left;"><span>But pause for a moment.</span></p><p style="text-align:left;"><span>Have you ever wondered what happens inside a paneer factory after those neatly packed paneer blocks leave for supermarkets, restaurants, and homes?</span></p><p style="text-align:left;"><span>For every kilogram of paneer produced, a large quantity of paneer whey is generated as residue. And while paneer brings revenue to dairy businesses, paneer whey often becomes one of the most difficult wastewater streams to manage.</span></p><p style="text-align:left;"><span>This is where the real story of dairy wastewater treatment begins.</span></p><p style="text-align:left;"><span><br/></span></p><h2 style="text-align:left;"><span>The Daily Challenge Most People Never See</span></h2><div><span><br/></span></div>
<p style="text-align:left;"><span>Paneer whey may look harmless at first. But inside dairy factories, it can quickly become a serious operational challenge if not handled properly.</span></p><p style="text-align:left;"><span>Paneer whey is highly biodegradable and contains a heavy organic load. According to treatment observations, paneer whey effluent can have:</span></p><p style="text-align:left;margin-left:36pt;"><span>·Inlet COD levels reaching up to 80,000 ppm</span></p><p style="text-align:left;margin-left:36pt;"><span>·Acidic pH around 4–5</span></p><p style="text-align:left;margin-left:36pt;"><span>·High protein content leading to sludge formation</span></p><p style="text-align:left;margin-left:36pt;"><span><br/></span></p><p style="text-align:left;"><span>At the same time, its high biodegradability also creates an opportunity for effective biological treatment when the right process is used.</span></p><p style="text-align:left;"><span>For dairy factory owners and MSMEs, the problem is rarely just “wastewater.” It slowly starts affecting operations, labour, hygiene, and even brand reputation.</span></p><h2 style="text-align:left;"><span>The Operational Pressure Behind Paneer Production</span></h2><div><span><br/></span></div>
<p style="text-align:left;"><span>Many dairy businesses silently struggle with issues like:</span></p><p style="text-align:left;"><span><br/></span></p><h3 style="text-align:left;"><span>Bad Odour and Fast Fermentation</span></h3><p style="text-align:left;"><span>Paneer whey spoils quickly when stored improperly.</span></p><p style="text-align:left;margin-left:36pt;"><span>·Fermentation can create strong unpleasant odours</span></p><p style="text-align:left;margin-left:36pt;"><span>·Nearby complaints may increase over time</span></p><p style="text-align:left;margin-left:36pt;"><span>·Working conditions inside the plant become uncomfortable</span></p><p style="text-align:left;margin-left:36pt;"><span><br/></span></p><h3 style="text-align:left;"><span>Drain Choking and Disposal Difficulties</span></h3><p style="text-align:left;"><span>The high organic nature of whey makes disposal challenging.</span></p><p style="text-align:left;margin-left:36pt;"><span>·Overflow and choking issues may occur</span></p><p style="text-align:left;margin-left:36pt;"><span>·Daily disposal becomes labour-intensive</span></p><p style="text-align:left;margin-left:36pt;"><span>·Transportation and handling costs keep increasing</span></p><p style="text-align:left;margin-left:36pt;"><span><br/></span></p><h3 style="text-align:left;"><span>Pollution and Compliance Concerns</span></h3><p style="text-align:left;"><span>Untreated or poorly treated wastewater can create environmental stress.</span></p><p style="text-align:left;margin-left:36pt;"><span>·Groundwater and nearby water sources may get affected</span></p><p style="text-align:left;margin-left:36pt;"><span>·Pollution control standards become difficult to maintain</span></p><p style="text-align:left;margin-left:36pt;"><span>·Frequent monitoring and corrective action may be required</span></p><p style="text-align:left;margin-left:36pt;"><span><br/></span></p><h3 style="text-align:left;"><span>Reputation and Business Impact</span></h3><p style="text-align:left;"><span>Today, word of mouth travels fast.</span></p><p style="text-align:left;margin-left:36pt;"><span>·Unhygienic surroundings can affect public perception</span></p><p style="text-align:left;margin-left:36pt;"><span>·Local complaints may damage trust slowly</span></p><p style="text-align:left;margin-left:36pt;"><span>·Operational stress starts affecting overall business focus</span></p><p style="text-align:left;"><span>Paneer brings profit.</span></p><p style="text-align:left;"><span>But unmanaged whey brings pressure.</span></p><p style="text-align:left;"><span>And most factory owners do not want unnecessary operational burdens standing between them and business growth.</span></p><p style="text-align:left;"><span><br/></span></p><h2 style="text-align:left;"><span>Why Traditional ETPs Sometimes Still Struggle</span></h2><p style="text-align:left;"><span>Many dairy factories already have some form of dairy effluent treatment plant installed.</span></p><p style="text-align:left;"><span>Yet in several cases, owners continue facing recurring issues.</span></p><p style="text-align:left;"><span>Not because they ignored treatment.</span></p><p style="text-align:left;"><span>But because paneer whey is a highly challenging wastewater stream.</span></p><p style="text-align:left;"><span>Conventional systems such as Activated Sludge Process (ASP) and MBBR systems often require:</span></p><p style="text-align:left;margin-left:36pt;"><span>·Continuous aeration through blowers</span></p><p style="text-align:left;margin-left:36pt;"><span>·Significant chemical usage</span></p><p style="text-align:left;margin-left:36pt;"><span>·Skilled operators for regular monitoring</span></p><p style="text-align:left;margin-left:36pt;"><span>·Secondary settling and sludge handling</span></p><p style="text-align:left;margin-left:36pt;"><span>·Higher recurring operational expenses</span></p><p style="text-align:left;"><br/></p><p style="text-align:left;"><span>In many cases, factories also experience:</span></p><p style="text-align:left;margin-left:36pt;"><span>·Persistent odour even after treatment</span></p><p style="text-align:left;margin-left:36pt;"><span>·Difficulty in handling high organic loads consistently</span></p><p style="text-align:left;margin-left:36pt;"><span>·Rising electricity consumption</span></p><p style="text-align:left;margin-left:36pt;"><span>·High sludge generation</span></p><p style="text-align:left;margin-left:36pt;"><span>·Unsatisfactory long-term operational experience</span></p><p style="text-align:left;"><span>For MSMEs especially, the challenge is not just installation.</span></p><p style="text-align:left;"><span>It is sustainability.</span></p><p style="text-align:left;"><span>A dairy ETP plant should not become another operational burden.</span></p><p style="text-align:left;"><span><br/></span></p><h2 style="text-align:left;"><span>So What Exactly Is an ETP?</span></h2><p style="text-align:left;"><span>An Effluent Treatment Plant (ETP) is a system designed to treat industrial wastewater before discharge or reuse.</span></p><p style="text-align:left;"><span>In paneer factories, ETPs help manage whey wastewater treatment by reducing pollutants, improving water quality, and supporting cleaner operations.</span></p><p style="text-align:left;"><span>A good paneer factory ETP should ideally help:</span></p><p style="text-align:left;margin-left:36pt;"><span>·Reduce organic load effectively</span></p><p style="text-align:left;margin-left:36pt;"><span>·Minimize odour issues</span></p><p style="text-align:left;margin-left:36pt;"><span>·Lower operational complexity</span></p><p style="text-align:left;margin-left:36pt;"><span>·Reduce recurring costs</span></p><p style="text-align:left;margin-left:36pt;"><span>·Improve treatment consistency</span></p><p style="text-align:left;margin-left:36pt;"><span>·Support pollution control compliance</span></p><p style="text-align:left;margin-left:36pt;"><span>·Enable treated water reuse wherever possible</span></p><p style="text-align:left;"><span>But not every ETP works the same way.</span></p><p style="text-align:left;"><span>The process design matters.</span></p><p style="text-align:left;"><span><br/></span></p><h2 style="text-align:left;"><span>A Different Approach to Paneer Whey Treatment</span></h2><p style="text-align:left;"><span>At Biofilm Engineers, the approach focuses on making dairy wastewater treatment simpler, cleaner, and operationally practical.</span></p><p style="text-align:left;"><span>The treatment flow includes:</span></p><p style="text-align:left;margin-left:36pt;"><span>·Pre-treatment</span></p><p style="text-align:left;margin-left:36pt;"><span>·Quorum Bio-reactor</span></p><p style="text-align:left;margin-left:36pt;"><span>·UV/Ozone polishing</span></p><p style="text-align:left;margin-left:36pt;"><span>·Treated water for reuse</span></p><p style="text-align:left;"><span>The system is designed to operate with:</span></p><p style="text-align:left;margin-left:36pt;"><span>·More than 90% operational expenditure reduction</span></p><p style="text-align:left;margin-left:36pt;"><span>·No chemical dependency during treatment</span></p><p style="text-align:left;margin-left:36pt;"><span>·Autonomous process control</span></p><p style="text-align:left;margin-left:36pt;"><span>·No secondary solids production</span></p><p style="text-align:left;"><span>One of the biggest differences lies in the reactor design itself.</span></p><p style="text-align:left;"><span>Unlike conventional submerged systems that rely heavily on artificial aeration, the Quorum Bio-reactor uses a non-submerged fixed film process with natural draft-based aeration.</span></p><p style="text-align:left;"><span>This helps reduce energy dependency significantly.</span></p><p style="text-align:left;"><span><br/></span></p><p style="text-align:left;"><img src="/Operational-savings.png"/><span></span></p><h2 style="text-align:left;"><br/></h2><h2 style="text-align:left;"><span>Real-World Operational Observation</span></h2><p style="text-align:left;"><span>In a 20 KLD paneer whey ETP installation at United Valley Foods India, Sullurupet, Andhra Pradesh, the following single-pass biofilter results were observed:</span></p><table border="0" cellspacing="0" cellpadding="0" style="text-align:left;"><thead><tr><td><p><span>Parameter</span></p></td><td><p><span>Inlet</span></p></td><td><p><span>Outlet</span></p></td><td><p><span>Removal</span></p></td></tr></thead><tbody><tr><td><p><span>pH</span></p></td><td><p><span>4.31</span></p></td><td><p><span>6.71</span></p></td><td><p><span>—</span></p></td></tr><tr><td><p><span>BOD (mg/L)</span></p></td><td><p><span>769</span></p></td><td><p><span>138</span></p></td><td><p><span>82.1%</span></p></td></tr><tr><td><p><span>COD (mg/L)</span></p></td><td><p><span>3637</span></p></td><td><p><span>560</span></p></td><td><p><span>84.6%</span></p></td></tr><tr><td><p><span>TSS (mg/L)</span></p></td><td><p><span>156</span></p></td><td><p><span>42</span></p></td><td><p><span>73.1%</span></p></td></tr></tbody></table><p style="text-align:left;"><span><br/></span></p><p style="text-align:left;"><span>The operational cost observed was less than INR 100/day.</span></p><p style="text-align:left;"><span>With a properly designed double-pass treatment approach, Pollution Control Board discharge norms can also be achieved more effectively for challenging paneer whey wastewater streams.</span></p><p style="text-align:left;"><span>These results indicate how biological process optimization can reduce treatment burden while maintaining practical operational efficiency.</span></p><p style="text-align:left;"><span><br/></span></p><h2 style="text-align:left;"><span>Why Biofilm-Based Systems Are Gaining Attention</span></h2><p style="text-align:left;"><span>Modern dairy wastewater treatment is gradually moving towards advanced biofilm technologies because of their higher microbial surface area and treatment efficiency.</span></p><p style="text-align:left;"><span>Compared to conventional systems:</span></p><p style="text-align:left;margin-left:36pt;"><span>·Conventional media may provide 500–5000 m²/m³ surface area</span></p><p style="text-align:left;margin-left:36pt;"><span>·Advanced biofilm carriers can provide extremely high microbial growth area</span></p><p style="text-align:left;margin-left:36pt;"><span>·Lower diffusion resistance improves treatment efficiency</span></p><p style="text-align:left;margin-left:36pt;"><span>·Natural oxygen transfer reduces blower dependency</span></p><p style="text-align:left;"><span>The result?</span></p><p style="text-align:left;"><span>Lower energy usage. Lower sludge generation. Lower chemical dependency. Simpler operations.</span></p><p style="text-align:left;"><span>For dairy MSMEs, these are not just technical advantages.</span></p><p style="text-align:left;"><span>They directly affect profitability, labour management, and long-term peace of mind.</span></p><p style="text-align:left;"><span><br/></span></p><h2 style="text-align:left;"><span>Cleaner Operations Are Becoming a Business Necessity</span></h2><p style="text-align:left;"><span>Today, dairy businesses are evolving quickly.</span></p><p style="text-align:left;"><span>Customers expect hygiene. Authorities expect compliance. Factories expect sustainability.</span></p><p style="text-align:left;"><span>And owners want systems that work quietly in the background without creating new operational headaches.</span></p><p style="text-align:left;"><span>The future of paneer whey treatment is not about fear.</span></p><p style="text-align:left;"><span>It is about responsible growth.</span></p><p style="text-align:left;"><span>Because a successful dairy business should focus more on producing quality products — not constantly worrying about wastewater disposal every single day.</span></p><p style="text-align:left;"><span><br/></span></p><h2 style="text-align:left;"><span>A Quiet Step Towards Better Dairy Operations</span></h2><p style="text-align:left;"><span>At Biofilm Engineers, the goal is simple.</span></p><p style="text-align:left;"><span>To help dairy factories move towards cleaner, calmer, and more manageable wastewater treatment systems through carefully designed biological treatment solutions.</span></p><p style="text-align:left;"><span>No exaggerated promises. No unnecessary complexity.</span></p><p style="text-align:left;"><span>Just practical engineering focused on operational comfort, lower recurring burden, and long-term treatment stability.</span></p><p style="text-align:left;"><span>Every dairy factory has its own challenges. Every wastewater stream behaves differently.</span></p><p style="text-align:left;"><span>But with the right treatment approach, paneer whey does not have to remain a daily operational pressure.</span></p><p style="text-align:left;"><span>Sometimes, the best industrial systems are the ones that quietly solve problems before they grow louder.</span></p><p style="text-align:left;"><span>And for many dairy businesses, that quiet change can make all the difference.</span></p><p style="text-align:left;"><span>&nbsp;</span></p><p style="text-align:left;"><span>&nbsp;</span></p></div><div style="text-align:left;"><br/></div><p></p></div>
</div></div></div></div></div><div data-element-id="elm_vmqzg4yotDdmtIcqZR6o8g" data-element-type="section" class="zpsection zpdefault-section zpdefault-section-bg "><style type="text/css"> [data-element-id="elm_vmqzg4yotDdmtIcqZR6o8g"].zpsection{ padding-block-end:99px; } </style><div class="zpcontainer-fluid zpcontainer"><div data-element-id="elm_37CB0NMxq-2dvE4qUJF4FQ" data-element-type="row" class="zprow zprow-container zpalign-items-flex-start zpjustify-content-flex-start zpdefault-section zpdefault-section-bg " data-equal-column="false"><style type="text/css"></style><div data-element-id="elm_nNUlocEzYcOz473k5aCrWQ" data-element-type="column" class="zpelem-col zpcol-12 zpcol-md-12 zpcol-sm-12 zpalign-self- zpdefault-section zpdefault-section-bg "><style type="text/css"></style><div data-element-id="elm_kNElefp6lO9Lplr7AEEGjw" data-element-type="video" class="zpelement zpelem-video "><style type="text/css"> @media (max-width: 767px) { [data-element-id="elm_kNElefp6lO9Lplr7AEEGjw"].zpelem-video iframe.zpvideo{ width:560px !important; height:315px !important; } } @media all and (min-width: 768px) and (max-width:991px){ [data-element-id="elm_kNElefp6lO9Lplr7AEEGjw"].zpelem-video iframe.zpvideo{ width:560px !important; height:315px !important; } } </style><div class="zpvideo-container zpiframe-align-center zpiframe-mobile-align-center zpiframe-tablet-align-center"><iframe class="zpvideo " width="750" height="500" src="//www.youtube.com/embed/DTqGLjNbpPo?enablejsapi=1" frameborder="0" allowfullscreen id=youtube-video-1 data-api=youtube></iframe></div>
</div></div></div></div></div></div> ]]></content:encoded><pubDate>Thu, 21 May 2026 10:53:52 +0000</pubDate></item><item><title><![CDATA[On Enhanced biological phosphorus removal]]></title><link>https://www.biofilmengineers.in/blogs/post/On-enhanced-biological-phosphorus-removal</link><description><![CDATA[<img align="left" hspace="5" src="https://www.biofilmengineers.in/enhanced biological phopshorus removal.JPG"/>Phosphorus accumulating organisms [PAOs] think different. They are not interested in crowd formation like organic heterotrophs. They know their niche. ]]></description><content:encoded><![CDATA[<div class="zpcontent-container blogpost-container "><div data-element-id="elm_3RRdF_huQBeP193BcL82JA" data-element-type="section" class="zpsection "><style type="text/css"></style><div class="zpcontainer-fluid zpcontainer"><div data-element-id="elm_eTbpUSvxTVmh1ngqo1t1JA" data-element-type="row" class="zprow zprow-container zpalign-items- zpjustify-content- " data-equal-column=""><style type="text/css"></style><div data-element-id="elm_ZsvetGgeS7atw6gQ88RXjw" data-element-type="column" class="zpelem-col zpcol-12 zpcol-md-12 zpcol-sm-12 zpalign-self- "><style type="text/css"> [data-element-id="elm_ZsvetGgeS7atw6gQ88RXjw"].zpelem-col{ border-radius:1px; } </style><div data-element-id="elm_T1fdGRmNSrq9WoZwilS2fw" data-element-type="text" class="zpelement zpelem-text "><style> [data-element-id="elm_T1fdGRmNSrq9WoZwilS2fw"].zpelem-text { border-radius:1px; } </style><div class="zptext zptext-align-center " data-editor="true"><div style="color:inherit;"><p style="text-align:justify;"><span style="color:inherit;">Phosphorus accumulating organisms [PAOs] think different.</span><br></p><p style="text-align:justify;"><span style="color:inherit;"><br></span></p><p style="text-align:justify;">They are not interested in crowd formation like organic heterotrophs. They know their niche. They know where their space is. They know themselves.</p><p style="text-align:justify;"><br></p><p style="text-align:justify;">Say, I’m a bacterium. I’m introduced to a new space/vast land with abundant resources for growth. The first strategy that comes to my mind is “I have to grow faster than others” [like our modern startups think]. They spend all their resources to outcompete their competitors. And at the end of the day, some do succeed. These are our typical ordinary organic heterotrophs that take organic carbon from the environment. They can double in 4 hours. And occupies most of the space available to them. Sounds great!</p><p style="text-align:justify;"><br></p><p style="text-align:justify;">But there’s this bacterium [the PAOs] that says, I’m not going to be an “ordinary heterotroph”. Fast-growing at the expense of resources, for me, is a primitive strategy. I know my niche. Let me do it my way.</p><p style="text-align:justify;"><br></p><p style="text-align:justify;">PAOs have a unique niche. It takes up organic carbon (in the form of volatile fatty acids) during the anaerobic period and stores it as polyhydroxyalkanoates [PHAs], by breaking down the polyphosphate [PP] polymers and glycogen reserves. While, during the aerobic period, it takes up the orthophosphate (PO<sub>4</sub>) in the water, at the expense of breaking the PHA. By doing this, it replenishes its glycogen and PP reserves. Indeed, think different.</p><p style="text-align:justify;"><span style="color:inherit;"><img src="/Mon%20Jun%2020%202022.png" alt=""></span><br></p><p style="text-align:justify;"><br></p><p style="text-align:justify;"><br></p><p style="text-align:justify;">Hope our modern-age bootstrapped startups learn from PAOs, the lesson to thrive in a competitive environment.</p><p style="text-align:justify;"><br></p><p style="text-align:justify;">P.S. I have written my Master's thesis based on EBPR. You may have a look.</p><p style="text-align:justify;"><span style="color:inherit;"><a href="https://odr.chalmers.se/bitstream/20.500.12380/301670/1/ACEX60%2C%20Ramesh%20Vasanth.pdf">https://odr.chalmers.se/bitstream/20.500.12380/301670/1/ACEX60%2C Ramesh Vasanth.pdf</a></span><br></p><p>&nbsp;</p></div></div>
</div></div></div></div></div></div> ]]></content:encoded><pubDate>Mon, 20 Jun 2022 07:01:30 +0000</pubDate></item><item><title><![CDATA[Why Anammox?]]></title><link>https://www.biofilmengineers.in/blogs/post/Why-anammox</link><description><![CDATA[<img align="left" hspace="5" src="https://www.biofilmengineers.in/anammox-FISH.PNG"/>]]></description><content:encoded><![CDATA[<div class="zpcontent-container blogpost-container "><div data-element-id="elm_qv5k7ZQuTwuMjF4uu8xZ8w" data-element-type="section" class="zpsection "><style type="text/css"></style><div class="zpcontainer-fluid zpcontainer"><div data-element-id="elm_XpBtyPb8RASOjm7ePfb5Iw" data-element-type="row" class="zprow zprow-container zpalign-items- zpjustify-content- " data-equal-column=""><style type="text/css"></style><div data-element-id="elm_eZPVebRKTiKj5GsXF0a9sg" data-element-type="column" class="zpelem-col zpcol-12 zpcol-md-12 zpcol-sm-12 zpalign-self- "><style type="text/css"> [data-element-id="elm_eZPVebRKTiKj5GsXF0a9sg"].zpelem-col{ border-radius:1px; } </style><div data-element-id="elm_voXf6jvBRIS1IOmjhiemKw" data-element-type="heading" class="zpelement zpelem-heading "><style> [data-element-id="elm_voXf6jvBRIS1IOmjhiemKw"].zpelem-heading { border-radius:1px; } </style><h2
 class="zpheading zpheading-style-none zpheading-align-center " data-editor="true"><div style="color:inherit;"><p style="text-align:justify;"><span style="font-size:13.5pt;color:inherit;">The following paragraphs are an excerpt from the breakthrough paper “Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor”.</span><br></p><p style="text-align:justify;"><span style="font-size:13.5pt;font-style:italic;text-decoration-line:underline;">Before Anammox - the problem:</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">Nitrogen removal from wastewater is critical for safeguarding sensitive water bodies. Nitrogen is often a rate-limiting compound for algae growth in surface water bodies. Nitrogen in wastewater when discharged into a water body, it makes it eutrophic. Eutrophication further leads to algae blooms.</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">Conventional biological nitrogen removal is a two-step process as discussed in FAQ-8. The first step is nitrification and the second step is the denitrification process. Nitrification in itself is often observed as a two-step process. The first step involves the conversion of ammonium into nitrite by ammonia-oxidizing bacteria/archaea. While the second step involves the conversion of nitrite to nitrate by nitrite-oxidizing bacteria. The first step of nitrification is a bottleneck, as the ammonium oxidizers are slow-growing autotrophs. And they compete with fast-growing heterotrophs for oxygen (electron acceptor). The denitrification process involves the conversion of produced nitrate to nitrogen gas. The bottleneck with the denitrification process is that it requires a carbon source (electron donor) and must be supplied. Nitrification and denitrification require different redox conditions i.e. aerobic and anoxic respectively. In wastewater treatment plants, separate chambers are required for each of these processes and the wastewater is cyclically pumped through these chambers.</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">Summary of the problems with conventional nitrification-denitrification:</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">1.<span style="font-size:7pt;">&nbsp;</span>High requirement of oxygen for nitrification reaction.</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">2.<span style="font-size:7pt;">&nbsp;</span>Requirement of carbon for denitrification reaction.</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">3.<span style="font-size:7pt;">&nbsp;</span>Needs a lot of space for constructing separate chambers and involves intensive pumping operations.</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;font-style:italic;text-decoration-line:underline;color:inherit;">Anammox the discovery:</span><br></p><p style="text-align:justify;"><span style="color:inherit;font-size:13.5pt;">In 1977,&nbsp; Ernest Broda stated, “Two kinds of litotrophs are missing in nature”.</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">He hypothesized that it must be possible to oxidize ammonium anaerobically with nitrite/nitrate as it’s thermodynamically feasible:</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">NH<sub>4</sub><sup>+</sup>&nbsp;+ NO<sub>2</sub><sup>-</sup>&nbsp;N<sub>2</sub>&nbsp;+ 2H<sub>2</sub>O &nbsp;ΔG = -358 kJ/mol NH<sub>4</sub><sup>+</sup></span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">Anammox (anaerobic ammonium oxidation) remained only in theory until a team of Dutch engineers and researchers observed an unusual pattern in their denitrifying fluidized bed reactor. The figure below shows the ammonium concentration in the influent. All was normal till day 420. From day 420, they started observing a significant “disappearance” of ammonium in the denitrifying reactor. The observation leads to the discovery of anammox. Now we know that anammox accounts for over 50% of nitrogen turnover in marine environments, forming a critical component of the global biogeochemical cycle.</span></p><p style="text-align:justify;"><img src="/anammox.PNG" style="width:526.5px;"><span style="font-size:13.5pt;"><br></span></p><p style="text-align:justify;"><span style="font-size:13.5pt;font-style:italic;text-decoration-line:underline;">Advantages of the Anammox process:</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;color:inherit;">1.</span><span style="color:inherit;font-size:7pt;">&nbsp;</span><span style="font-size:13.5pt;color:inherit;">Lower energy consumption – as the aeration required is reduced.</span><br></p><div><p style="text-align:justify;"><span style="font-size:13.5pt;">2.<span style="font-size:7pt;">&nbsp;</span>No external carbon is required.</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">3.<span style="font-size:7pt;">&nbsp;</span>Lower footprint. Complete nitrogen removal can be performed in a single bioreactor.</span></p></div><p style="text-align:justify;"><span style="font-size:13.5pt;font-style:italic;"><br></span></p><p style="text-align:justify;"><img src="/anammox_flow.PNG" style="width:621.8px;"><span style="font-size:13.5pt;"><br></span></p><p style="text-align:justify;"><img src="/anammox-FISH.PNG"><span style="font-size:13.5pt;"><br></span></p><p style="text-align:justify;"><span style="font-style:italic;font-size:18px;">Credits: To my Guru, Prof. Frank Persson, Chalmers University of Technology, Sweden.</span><br></p><p style="margin-left:18pt;text-align:justify;"><span style="font-size:13.5pt;">&nbsp;</span></p><p style="text-align:justify;"><span style="font-size:13.5pt;">&nbsp;</span></p></div></h2></div>
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</div></div></div></div></div></div> ]]></content:encoded><pubDate>Sun, 19 Jun 2022 08:29:08 +0000</pubDate></item></channel></rss>